| Bio::Draw::Pictogram(3pm) | User Contributed Perl Documentation | Bio::Draw::Pictogram(3pm) |
Bio::Draw::Pictogram - generate SVG output of Pictogram display for consensus motifs
use Bio::Draw::Pictogram;
use Bio::SeqIO;
my $sio = Bio::SeqIO->new(-file=>$ARGV[0],-format=>'fasta');
my @seq;
while(my $seq = $sio->next_seq){
push @seq, $seq;
}
my $picto = Bio::Draw::Pictogram->new(-width=>"800",
-height=>"500",
-fontsize=>"60",
-plot_bits=>1,
-background=>{
'A'=>0.25,
'C'=>0.18,
'T'=>0.32,
'G'=>0.25},
-color=>{'A'=>'red',
'G'=>'blue',
'C'=>'green',
'T'=>'magenta'});
my $svg = $picto->make_svg(\@seq);
print $svg->xmlify."\n";
#Support for Bio::Matrix::PSM::SiteMatrix now included
use Bio::Matrix::PSM::IO;
my $picto = Bio::Draw::Pictogram->new(-width=>"800",
-height=>"500",
-fontsize=>"60",
-plot_bits=>1,
-background=>{
'A'=>0.25,
'C'=>0.18,
'T'=>0.32,
'G'=>0.25},
-color=>{'A'=>'red',
'G'=>'blue',
'C'=>'green',
'T'=>'magenta'});
my $psm = $psmIO->next_psm;
my $svg = $picto->make_svg($psm);
print $svg->xmlify;
A module for generating SVG output of Pictogram display for consensus motifs. This method of representation was describe by Burge and colleagues: (Burge, C.B.,Tuschl, T., Sharp, P.A. in The RNA world II, 525-560, CSHL press, 1999)
This is a simple module that takes in an array of sequences (assuming equal lengths) and calculates relative base frequencies where the height of each letter reflects the frequency of each nucleotide at a given position. It can also plot the information content at each position scaled by the background frequencies of each nucleotide.
It requires the SVG-2.26 or later module by Ronan Oger available at http://www.cpan.org
Recommended viewing of the SVG is the plugin available at Adobe: http://www.adobe.com/svg
User feedback is an integral part of the evolution of this and other Bioperl modules. Send your comments and suggestions preferably to one of the Bioperl mailing lists. Your participation is much appreciated.
bioperl-l@bioperl.org - General discussion http://bioperl.org/wiki/Mailing_lists - About the mailing lists
Please direct usage questions or support issues to the mailing list:
bioperl-l@bioperl.org
rather than to the module maintainer directly. Many experienced and reponsive experts will be able look at the problem and quickly address it. Please include a thorough description of the problem with code and data examples if at all possible.
Report bugs to the Bioperl bug tracking system to help us keep track the bugs and their resolution. Bug reports can be submitted via the web:
https://github.com/bioperl/bioperl-live/issues
Email shawnh@fugu-sg.org
The rest of the documentation details each of the object methods. Internal methods are usually preceded with a "_".
Title : new
Usage : my $picto = Bio::Draw::Pictogram->new(-width=>"800",
-height=>"500",
-fontsize=>"60",
-plot_bits=>1,
-background=>{
'A'=>0.25,
'C'=>0.18,
'T'=>0.32,
'G'=>0.25},
-color=>{'A'=>'red',
'G'=>'blue',
'C'=>'green',
'T'=>'magenta'});
Function: Constructor for Pictogram Object
Returns : L<Bio::Draw::Pictogram>
Title : make_svg Usage : $picto->make_svg(); Function: make the SVG object Returns : L<SVG> Arguments: A fasta file or array ref of L<Bio::Seq> objects or a L<Bio::Matrix::PSM::SiteMatrixI>
Title : fontsize Usage : $picto->fontsize(); Function: get/set for fontsize Returns : int Arguments: int
Title : color Usage : $picto->color(); Function: get/set for color Returns : a hash reference Arguments: a hash reference
Title : svg_obj Usage : $picto->svg_obj(); Function: get/set for svg_obj Returns : L<SVG> Arguments: L<SVG>
Title : plot_bits
Usage : $picto->plot_bits();
Function: get/set for plot_bits to indicate whether to plot
information content at each base position
Returns :1/0
Arguments: 1/0
Title : normalize
Usage : $picto->normalize($newval)
Function: get/set to make all columns the same height.
default is to scale height with information
content.
Returns : value of normalize (a scalar)
Args : on set, new value (a scalar or undef, optional)
Title : background Usage : $picto->background(); Function: get/set for hash reference of nucleodtide bgd frequencies Returns : hash reference Arguments: hash reference
Title : pwm Usage : $picto->pwm(); Function: get/set for pwm Returns : int Arguments: int
| 2018-10-27 | perl v5.26.2 |