NAME

alifmt - Aligned sequences formats

DESCRIPTION

This document illustrates some common formats used for aligned sequences representation.

CLUSTAL

 CLUSTAL W (1.82) multiple sequence alignment
 MALK_ECOLI      MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKSTL
 MALK_SALTY      MASVQLRNVTKAWGDVVVSKDINLDIHDGEFVVFVGPSGCGKSTL
 MALK_ENTAE      MASVQLRNVTKAWGDVVVSKDINLEIQDGEFVVFVGPSGCGKSTL
 MALK_PHOLU      MSSVTLRNVSKAYGETIISKNINLEIQEGEF--------------
                 *:** *:**:**:*:.::**:***:*::***
 MALK_ECOLI      LRMIAGLETITSGDLACRRLHKEPGV
 MALK_SALTY      LRMIAGLETITSGDLACRRLHQEPGV
 MALK_ENTAE      LRMIAGLETVTSGDL-----------
 MALK_PHOLU      LRM-----------------------
                 ***
    

Warning: Names must not contain spaces or exceed 30 characters.

FASTA

 >MALK_ECOLI
 MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKSTLLRMIA
 GLETITSGDLACRRLHKEPGV
 >MALK_SALTY
 MASVQLRNVTKAWGDVVVSKDINLDIHDGEFVVFVGPSGCGKSTLLRMIA
 GLETITSGDLACRRLHQEPGV
 >MALK_ENTAE
 MASVQLRNVTKAWGDVVVSKDINLEIQDGEFVVFVGPSGCGKSTLLRMIA
 GLETVTSGDL-----------
 >MALK_PHOLU
 MSSVTLRNVSKAYGETIISKNINLEIQEGEF--------------LRM--
 ---------------------
    

MEGA

 #mega
 !Title Multiple Sequence Alignment;
 #MALK_ECOLI
 MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKSTLLRMIA
 GLETITSGDLACRRLHKEPGV
 #MALK_SALTY
 MASVQLRNVTKAWGDVVVSKDINLDIHDGEFVVFVGPSGCGKSTLLRMIA
 GLETITSGDLACRRLHQEPGV
 #MALK_ENTAE
 MASVQLRNVTKAWGDVVVSKDINLEIQDGEFVVFVGPSGCGKSTLLRMIA
 GLETVTSGDL-----------
 #MALK_PHOLU
 MSSVTLRNVSKAYGETIISKNINLEIQEGEF-------------------
 ---------------------
    

MSF

 !!AA_MULTIPLE_ALIGNMENT 1.0
 PileUp of: @pep.list
  msf.seq       MSF: 55  Type: P  Nov 22, 2001 11:02  Check: 2529 ..
  Name: m73237           Len:   655  Check: 7493  Weight:  1.00
  Name: l28824           Len:   655  Check: 5456  Weight:  1.00
  Name: u04379           Len:   655  Check: 9580  Weight:  1.00
 //
         1                                                   50
 m73237  ~~~~~MADSA NHLPFFFGQI TREEAEDYLV QGGMSDGLYL LRQSRNYLGG
 l28824  MASSGMADSA NHLPFFFGNI TREEAEDYLV QGGMSDGLYL LRQSRNYLGG
 u04379  ~~~~~MPDPA AHLPFFYGSI SRAEAEEHLK LAGMADGLFL LRQCLRSLGG
         51
 m73237  ~~~~~
 l28824  ~~~~~
 u04379  AACG*
    

Warning: This format cannot handle more than 500 sequences in a single alignment.

NEXUS

 #NEXUS
 begin data;
   dimensions ntax=2 nchar=80;
   format datatype=Protein interleave gap=- missing='.';
   matrix
 [           1                                               50]
 btdDm       -------AQQQQHHLHMQQAQHH-----------LHLSH------QQAQQ
 TSp1        --------------------AEH-----------PSLR--------GTPL
 [           51                          80]
 btdDm       YACPICSKKFSRSDHLSKHKKTHF------
 TSp1        FACPICNKRFMRSDHLAKHVKTHN------
     ;
 endblock;
    

Warning: Text enclosed in brackets is considered as comment, and thus ignored.

PHYLIP

Sequential (PHYLIPS):

      2   109
 ATISA1    GSPNLYQ-GGRKPWHSINFICAHDGFTLADLVTYNNKNNLANGEENNDG
           ENHNYSWNCGEEGDFASISVKRLRKRQMRNFFVSLMVSQGVPMIYMGDE
           YGHTKGGN---
 POTISA1   GSPNLYQKGGRKPWNSINFVCAHDGFTLADLVTYNNKHNLANGEDNKDG
           ENHNNSWNCGEEGEFASIFVKKLRKRQMRNFFLCLMVSQGVPMIYMGDE
           YGHTKGGN---
    

Interleaved (PHYLIPI):

      2   109
 ATISA1    GSPNLYQ-GGRKPWHSINFICAHDGFTLADLVTYNNKNNLANGEENND
 POTISA1   GSPNLYQKGGRKPWNSINFVCAHDGFTLADLVTYNNKHNLANGEDNKD
           GENHNYSWNCGEEGDFASISVKRLRKRQMRNFFVSLMVSQGVPMIYMG
           GENHNNSWNCGEEGEFASIFVKKLRKRQMRNFFLCLMVSQGVPMIYMG
           DEYGHTKGGN---
           DEYGHTKGGN---
    

Warning: Species names may not contain characters `( ) : ; , [ ]' and exceed 10 characters.

STOCKHOLM

 # STOCKHOLM 1.0
 MALK_ECOLI  MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKSTLLRMIA
 MALK_SALTY  MASVQLRNVTKAWGDVVVSKDINLDIHDGEFVVFVGPSGCGKSTLLRMIA
 MALK_ENTAE  MASVQLRNVTKAWGDVVVSKDINLEIQDGEFVVFVGPSGCGKSTLLRMIA
 MALK_PHOLU  MSSVTLRNVSKAYGETIISKNINLEIQEGEF-------------------
 MALK_ECOLI  RCHLFREDGTACRRLHKEPGV
 MALK_SALTY  RCHLFREDGSACRRLHQEPGV
 MALK_ENTAE  ---------------------
 MALK_PHOLU  ---------------------
 //
    

SEE ALSO

squizz(1), seqfmt(5)

AUTHOR

Nicolas Joly (njoly@pasteur.fr), Institut Pasteur.