help:

--version

show program's version number and exit

-h, --help

show this help message and exit

subcommands:

Sequence file operation

count

Splits sequences files by number of records.

group

Splits sequences files by annotation.

sample

Randomly samples from unpaired sequences files.

samplepair

Randomly samples from paired-end sequences files.

sort

Sorts sequences files by annotation.

select

Selects sequences from sequence files by annotation.

output files:

part<part>

reads partitioned by count, where <part> is the partition number.

<field>-<value>

reads partitioned by annotation <field> and <value>.

under-<number>

reads partitioned by numeric threshold where the annotation value is strictly less than the threshold <number>.

atleast-<number>

reads partitioned by numeric threshold where the annotation value is greater than or equal to the threshold <number>.

sorted

reads sorted by annotation value.

sorted-part<part>

reads sorted by annotation value and partitioned by count, where <part> is the partition number.

sample<i>-n<count>

randomly sampled reads where <i> is a number specifying the sampling instance and <count> is the number of sampled reads.

selected

reads passing selection criteria.

output annotation fields:

None