| Bio::DB::Query::GenBank(3pm) | User Contributed Perl Documentation | Bio::DB::Query::GenBank(3pm) |
Bio::DB::Query::GenBank - Build a GenBank Entrez Query
use Bio::DB::Query::GenBank;
use Bio::DB::GenBank;
my $query_string = 'Oryza[Organism] AND EST[Keyword]';
my $query = Bio::DB::Query::GenBank->new(-db => 'nucleotide',
-query => $query_string,
-mindate => '2001',
-maxdate => '2002');
print $query->count,"\n";
# get a Genbank database handle
my $gb = Bio::DB::GenBank->new();
my $stream = $gb->get_Stream_by_query($query);
while (my $seq = $stream->next_seq) {
# do something with the sequence object
}
# initialize the list yourself
my $query = Bio::DB::Query::GenBank->new(-ids=>[195052,2981014,11127914]);
This class encapsulates NCBI Entrez queries. It can be used to store a list of GI numbers, to translate an Entrez query expression into a list of GI numbers, or to count the number of terms that would be returned by a query. Once created, the query object can be passed to a Bio::DB::GenBank object in order to retrieve the entries corresponding to the query.
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The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _
Title : new
Usage : $db = Bio::DB::Query::GenBank->new(@args)
Function: create new query object
Returns : new query object
Args : -db database (see below for allowable values)
-query query string
-mindate minimum date to retrieve from (YYYY/MM/DD)
-maxdate maximum date to retrieve from (YYYY/MM/DD)
-reldate relative date to retrieve from (days)
-datetype date field to use ('edat' or 'mdat')
-ids array ref of gids (overrides query)
-maxids the maximum number of IDs you wish to collect
(defaults to 100)
-email Email address; required if you want to decrease
delay time between queries
-delay Delay time (in seconds). Note NCBI policy requires 4
seconds between requests unless an email is provided
This method creates a new query object. Typically you will specify a -db and a -query argument, possibly modified by -mindate, -maxdate, or -reldate. -mindate and -maxdate specify minimum and maximum dates for entries you are interested in retrieving, expressed in the form YYYY/MM/DD. -reldate is used to fetch entries that are more recent than the indicated number of days.
If you provide an array reference of IDs in -ids, the query will be ignored and the list of IDs will be used when the query is passed to a Bio::DB::GenBank object's get_Stream_by_query() method. A variety of IDs are automatically recognized, including GI numbers, Accession numbers, Accession.version numbers and locus names.
By default, the query will collect only the first 100 IDs and will generate an exception if you call the ids() method and the query returned more than that number. To increase this maximum, set -maxids to a number larger than the number of IDs you expect to obtain. This only affects the list of IDs you obtain when you call the ids() method, and does not affect in any way the number of entries you receive when you generate a SeqIO stream from the query.
-db option values:
The most commonly used databases are:
protein
nucleotide
nuccore
nucgss
nucest
unigene
An up to date list of database names supported by NCBI eUtils is
always available at:
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/einfo.fcgi?
However, note that not all of these databases return datatypes that
are parsable by Bio::DB::GenBank
Title : cookie Usage : ($cookie,$querynum) = $db->cookie Function: return the NCBI query cookie Returns : list of (cookie,querynum) Args : none
NOTE: this information is used by Bio::DB::GenBank in conjunction with efetch.
Title : _request_parameters Usage : ($method,$base,@params = $db->_request_parameters Function: return information needed to construct the request Returns : list of method, url base and key=>value pairs Args : none
Title : count Usage : $count = $db->count; Function: return count of number of entries retrieved by query Returns : integer Args : none
Returns the number of entries that are matched by the query.
Title : ids Usage : @ids = $db->ids([@ids]) Function: get/set matching ids Returns : array of sequence ids Args : (optional) array ref with new set of ids
Title : query Usage : $query = $db->query([$query]) Function: get/set query string Returns : string Args : (optional) new query string
Title : _parse_response Usage : $db->_parse_response($content) Function: parse out response Returns : empty Args : none Throws : 'unparseable output exception'
Title : _generate_id_string Usage : $string = $db->_generate_id_string Function: joins IDs together in string (possibly implementation-dependent) Returns : string of concatenated IDs Args : array ref of ids (normally passed into the constructor)
Title : delay_policy
Usage : $secs = $self->delay_policy
Function: NCBI requests a delay of 4 seconds between requests unless email is
provided. This method implements a 4 second delay; use 'delay()' to
override, though understand if no email is provided we are not
responsible for users being IP-blocked by NCBI
Returns : number of seconds to delay
Args : none
| 2022-02-24 | perl v5.34.0 |