DOKK / manpages / debian 12 / libbio-perl-perl / Bio::Align::PairwiseStatistics.3pm.en
Bio::Align::PairwiseStatistics(3pm) User Contributed Perl Documentation Bio::Align::PairwiseStatistics(3pm)

Bio::Align::PairwiseStatistics - Base statistic object for Pairwise Alignments

  use strict;
  my $stats = Bio::Align::PairwiseStatistics->new();
  # get alignment object of two sequences somehow
  my $pwaln;
  print $stats->number_of_comparable_bases($pwaln);
  my $score = $stats->score_nuc($pwaln);

Calculate pairwise statistics.

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The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _

 Title   : number_of_comparable_bases
 Usage   : my $bases = $stat->number_of_comparable_bases($aln);
 Function: Returns the count of the number of bases that can be
           compared (L) in this alignment ( length - gaps)
 Returns : integer
 Args    : L<Bio::Align::AlignI>

 Title   : number_of_differences
 Usage   : my $nd = $stat->number_of_distances($aln);
 Function: Returns the number of differences between two sequences
 Returns : integer
 Args    : L<Bio::Align::AlignI>

 Title   : number_of_gaps
 Usage   : my $nd = $stat->number_of_gaps($aln);
 Function: Returns the number of gapped positions among sequences in alignment
 Returns : integer
 Args    : L<Bio::Align::AlignI>

 Title   : score_nuc
 Usage   : my $score = $stat->score_nuc($aln);
             or
           my $score = $stat->score_nuc(
             -aln =>$aln,
             -match    => 1,
             -mismatch => -1,
             -gap_open => -1,
             -gap_ext  => -1
           );
 Function: Calculate the score of an alignment of 2 nucleic acid sequences. The
           scoring parameters can be specified. Otherwise the blastn default
           parameters are used: match = 2, mismatch = -3, gap opening = -5, gap
           extension = -2
 Returns : alignment score (number)
 Args    : L<Bio::Align::AlignI>
           match score [optional]
           mismatch score [optional]
           gap opening score [optional]
           gap extension score [optional]
2021-08-15 perl v5.32.1