macs2_bdgopt - Model-based Analysis for ChIP-Sequencing
usage: macs2 bdgopt [-h] -i IFILE [-m
{multiply,add,p2q,max,min}]
- [-p [EXTRAPARAM ...]] [--outdir OUTDIR] -o OFILE
- -h, --help
- show this help message and exit
- -i IFILE, --ifile
IFILE
- MACS score in bedGraph. Note: this must be a bedGraph file covering the
ENTIRE genome. REQUIRED
- -m
{multiply,add,p2q,max,min}, --method
{multiply,add,p2q,max,min}
- Method to modify the score column of bedGraph file. Available choices are:
multiply, add, max, min, or p2q. 1) multiply, the EXTRAPARAM is required
and will be multiplied to the score column. If you intend to divide the
score column by X, use value of 1/X as EXTRAPARAM. 2) add, the EXTRAPARAM
is required and will be added to the score column. If you intend to
subtract the score column by X, use value of -X as EXTRAPARAM. 3)
max, the EXTRAPARAM is required and will take the maximum value between
score and the EXTRAPARAM. 4) min, the EXTRAPARAM is required and will take
the minimum value between score and the EXTRAPARAM. 5) p2q, this will
convert p-value scores to q-value scores using Benjamini-Hochberg process.
The EXTRAPARAM is not required. This method assumes the scores are
-log10 p-value from MACS2. Any other types of score will cause
unexpected errors.
- -p [EXTRAPARAM ...],
--extra-param [EXTRAPARAM ...]
- The extra parameter for METHOD. Check the detail of -m option.
- --outdir
OUTDIR
- If specified all output files will be written to that directory. Default:
the current working directory
- -o OFILE, --ofile
OFILE
- Output BEDGraph filename.