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ncbi-blast+(1) USER COMMANDS ncbi-blast+(1)

ncbi-blast+ - NCBI command tools for BLAST+

BLAST+ documentation is available online at http://www.ncbi.nlm.nih.gov/books/NBK1763/

The NCBI BLAST+ is a group of tools for basic alignment search. BLAST+
is a rewrite of BLAST with new features and performance improvements. BLAST+ commands differ from original BLAST. For BLAST users, one should read
the BLAST USERS paragraph.

BLAST+ uses the BLASTDB environment variable to get default db directory. It is possible to update the file /etc/ncbi/.ncbirc to add the following: [BLAST] BLASTDB=/var/lib/ncbi-blast+/db

The easiest way to get started using these command line applications is
by means of the legacy_blast.pl PERL script which is bundled along with
the BLAST+ applications. To utilize this script, simply prefix it to the invocation of the C toolkit
BLAST command line application and append the --path option pointing to the installation directory of the BLAST+ applications. For example, instead of using

blastall -i query -d nr -o blast.out use legacy_blast.pl blastall -i query -d nr -o blast.out --path /usr/bin

Olivier Sallou (olivier.sallou (at) irisa.fr) - Man page and packaging NCBI - BLAST+

April 29, 2011 version 2.2.25