| SICKLE(1) | User Commands | SICKLE(1) |
sickle - windowed adaptive trimming tool for FASTQ files using quality
sickle <command> [options]
## Usage
Sickle has two modes to work with both paired-end and single-end reads: `sickle se` and `sickle pe`.
Running sickle by itself will print the help:
sickle
Running sickle with either the "se" or "pe" commands will give help specific to those commands:
sickle se
sickle pe
### Sickle Single End (`sickle se`)
`sickle se` takes an input fastq file and outputs a trimmed version of that file. It also has options to change the length and quality thresholds for trimming, as well as disabling 5'-trimming and enabling truncation of sequences with Ns.
#### Examples
sickle se -f input_file.fastq -t illumina -o trimmed_output_file.fastq
sickle se -f input_file.fastq -t illumina -o trimmed_output_file.fastq -q 33
-l 40
sickle se -f input_file.fastq -t illumina -o trimmed_output_file.fastq -x -n
sickle se -t sanger -g -f input_file.fastq -o trimmed_output_file.fastq.gz
sickle se --fastq-file input_file.fastq --qual-type sanger --output-file
trimmed_output_file.fastq
### Sickle Paired End (`sickle pe`)
`sickle pe` can operate with two types of input. First, it can take two paired-end files as input and outputs two trimmed paired-end files as well as a "singles" file. The second form starts with a single combined input file of reads where you have already interleaved the reads from the sequencer. In this form, you also supply a single output file name as well as a "singles" file. The "singles" file contains reads that passed filter in either the forward or reverse direction, but not the other. Finally, there is an option (-M) to only produce one interleaved output file where any reads that did not pass filter will be output as a FastQ record with a single "N" (whose quality value is the lowest possible based upon the quality type), thus preserving the paired nature of the data. You can also change the length and quality thresholds for trimming, as well as disable 5'-trimming and enable truncation of sequences with Ns.
#### Examples
sickle pe -f input_file1.fastq -r input_file2.fastq -t sanger -o
trimmed_output_file1.fastq -p trimmed_output_file2.fastq -s
trimmed_singles_file.fastq
sickle pe -f input_file1.fastq -r input_file2.fastq -t sanger -o
trimmed_output_file1.fastq -p trimmed_output_file2.fastq -s
trimmed_singles_file.fastq -q 12 -l 15
sickle pe -f input_file1.fastq -r input_file2.fastq -t sanger -o
trimmed_output_file1.fastq -p trimmed_output_file2.fastq -s
trimmed_singles_file.fastq -n
sickle pe -c combo.fastq -t sanger -m combo_trimmed.fastq -s
trimmed_singles_file.fastq -n
sickle pe -t sanger -g -f input_file1.fastq -r input_file2.fastq -o
trimmed_output_file1.fastq.gz -p trimmed_output_file2.fastq.gz -s
trimmed_singles_file.fastq.gz
sickle pe -c combo.fastq -t sanger -M combo_trimmed_all.fastq
sickle pe --pe-file1 input_file1.fastq --pe-file2 input_file2.fastq
--qual-type sanger --output-pe1 trimmed_output_file1.fastq --output-pe2
trimmed_output_file2.fastq --output-single trimmed_singles_file.fastq
Command: pe paired-end sequence trimming se single-end sequence trimming
--help, display this help and exit --version, output version information and exit
Written by Nikhil Joshi, UC Davis Bioinformatics Core
Copyright © 2011 The Regents of University of California, Davis Campus. sickle is free software and comes with ABSOLUTELY NO WARRANTY. Distributed under the MIT License.
The full documentation for sickle is maintained as a Texinfo manual. If the info and sickle programs are properly installed at your site, the command
should give you access to the complete manual.
| June 2020 | sickle version 1.33 |